Molecular Tests

The molecular tests offered by BC CAHS are based on the detection of nucleic acid sequences using PCR based methods. These include: classical Polymerase Chain Reaction (PCR), Quantitative PCR (qPCR), and Reverse Transcription qPCR (RT-qPCR).

BC CAHS currently offers diagnostic tests for the following pathogens:

 

  • A.sal, Aeromonas salmonicida
  • IHNv, Infectious hematopoetic necrosis virus
  • IPNv, Infectious pancreatic necrosis virus
  • ISAv, Infectious salmon anemia virus 
  • N.perur, Neoparamoeba perurans
  • PRv, Piscine reovirus 
  • P.sal, Piscirickettsia salmonis
  • R.sal, Renibacterium salmoninarum 
  • SAV, Salmonid alphavirus
  • V.para, Vibrio parahaemolyticus
  • P.pseudo, Parvicapsula pseudobranchicola
  • VHSv, Viral hemorrhagic septicemia virus 
  • Y.ruck, Yersina ruckeri
  • Kudoa thyrsites
  • Whirling Disease, Myxobolus cerebralis

 

Molecular methods (PCR, Quantitative PCR and Reverse Transcription-qPCR) are commonly used at BC CAHS to screen for the presence/absence of pathogens using specific nucleic acid sequences. From this, inferences about the presence of disease-causing agents can made. The sensitivity of the molecular assays at BC CAHS allows for a variety of sample sizes that can be submitted for testing.

 

These molecular methods can be used to help answer a wide range of biological questions, and BC CAHS endeavours to stay at the forefront of developing needs.  BC CAHS has the in-house expertise to work with clients to develop additional methods for the detection of emerging pathogens or any organism of interest. Please contact us to discuss your needs.